BoNT/A1 (D356)

Sequence

BLAST     FASTA    
MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDLNKGEEITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPNIERFPNGKKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTFFSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIGNMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRNEKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDLSSKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRGTLIGQVDRLKDKVNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPIDKNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIWIKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKGPRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAKLVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL

Properties

MW: 149,426.89 Da
No. of AA: 1296
Abs 0.1% (g/L): 1.292
pI: 6.06
NCBI ID: WP_011948511.1
UniprotKB: P0DPI1
Strains: CDC 1690, NCTC 7272, CDC36923, CDC220727, 32A, VPI 7124, Nan 2006, 33A, H114590007, AM1295, NCTC 3805, 4979, KF Meyer 126, 96A, KF Meyer 33, Prevot 697B, CN 7236, McClung 844, CDC 21547, DFPST0029, ATCC 19397, 39 KA, CDC220726, H114580651, H114590003, 1192A, 62A, Hall A-hyper, ATCC 3502, A Hall, NCTC 3806, Allergan-Hall A, Cam2A, FT30, NCTC 13319, CN 5008, H114720640, NCTC 387, CDC36955, 69A, 16037, ATCC 449, ATCC 25763, CDC36956, Hall, 765, ATCC 17862, Hall A, H114580654, Prevot Dewping, 887, CDC36924, NCTC 4587, BS-A, Prevot 910, 73A, 213, VL1, NCTC 3805 (2), IFR 18/076, IFR 18/027, NCTC 7272 (3), IFR 18/142, 62A (3), 397/5, 62A (2), IFR 18/105, IFR 18/107, NCTC 7272 (2), IFR 16/362, NCTC 7272 (4), IFR 18/091, NCTC 3806 (2)
Associated Proteins: BoNT/A1 (ADF1).
Notes:

Associated Publications

Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes.
Sebaihia M, Peck MW, Minton NP, Thomson NR, Holden MT, Mitchell WJ, Carter AT, Bentley SD, Mason DR, Crossman L, Paul CJ, Ivens A, Wells-Bennik MH, Davis IJ, Cerdeno-Tarraga AM, Churcher C, Quail MA, Chillingworth T, Feltwell T, Fraser A, Goodhead I, Hanc (2007). Genome Res.
DOI: 10.1101/gr.6282807   PMID: 17519437

Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids.
Smith TJ, Hill KK, Foley BT, Detter JC, Munk AC, Bruce DC, Doggett NA, Smith LA, Marks JD, Xie G, Brettin TS. (2007). PLoS ONE
DOI: 10.1371/journal.pone.0001271   PMID: 18060065

Characterization of botulinum type A neurotoxin gene: delineation of the N-terminal encoding region.
Betley MJ, Somers E, DasGupta BR. (1989). Biochem. Biophys. Res. Commun.
DOI:   PMID: 2669749

Botulinum type A neurotoxin digested with pepsin yields 132, 97, 72, 45, 42, and 18 kD fragments.
Gimenez JA, DasGupta BR. (1993). J. Protein Chem.
DOI:   PMID: 8397793

The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A Subtypes.
Pellett S, Bradshaw M, Tepp WH, Pier CL, Whitemarsh RCM, Chen C, Barbieri JT, Johnson EA. (2018). MBio
DOI: 10.1128/mBio.00089-18   PMID: 29588398

High resolution crystal structures of the receptor-binding domain of neurotoxin serotypes A and FA.
Davies JR, Hackett GS, Liu SM, Acharya KR. (2018). PeerJ
DOI: 10.7717/peerj.4552   PMID: 29576992

Comparison of efficacy and toxicity between botulinum toxin subtypes A1 and A2 in cynomolgus macaques.
Torii Y, Sasaki M, Shin MC, Akaike N, Kaji R. (2018). Toxicon
DOI: 10.1016/j.toxicon.2018.08.017   PMID: 30193802

Persistence of botulinum neurotoxin a subtypes 1-5 in primary rat spinal cord cells.
Whitemarsh RC, Tepp WH, Johnson EA, Pellett S. (2014). PLoS ONE
DOI: 10.1371/journal.pone.0090252   PMID: 24587301

Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons.
Joussain C, Le Coz O, Pichugin A, Marconi P, Lim F, Sicurella M, Salonia A, Montorsi F, Wandosell F, Foster K, Giuliano F, Epstein AL, Aranda Muñoz A. (2019). Toxins (Basel)
DOI: 10.3390/toxins11020123   PMID: 30791373

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