BoNT/B1 (D145)

Sequence

BLAST     FASTA    
MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKEHLAVYKIQMCKSVKAPGICIDVDNEDLFFIADKNSFSDDLSKNERIEYNTQSNYIENDFPINELILDTDLISKIELPSENTESLTDFNVDVPVYEKQPAIKKIFTDENTIFQYLYSQTFPLDIRDISLTSSFDDALLFSNKVYSFFSMDYIKTANKVVEAGLFAGWVKQIVNDFVIEANKSNTMDKIADISLIVPYIGLALNVGNETAKGNFENAFEIAGASILLEFIPELLIPVVGAFLLESYIDNKNKIIKTIDNALTKRNEKWSDMYGLIVAQWLSTVNTQFYTIKEGMYKALNYQAQALEEIIKYRYNIYSEKEKSNINIDFNDINSKLNEGINQAIDNINNFINGCSVSYLMKKMIPLAVEKLLDFDNTLKKNLLNYIDENKLYLIGSAEYEKSKVNKYLKTIMPFDLSIYTNDTILIEMFNKYNSEILNNIILNLRYKDNNLIDLSGYGAKVEVYDGVELNDKNQFKLTSSANSKIRVTQNQNIIFNSVFLDFSVSFWIRIPKYKNDGIQNYIHNEYTIINCMKNNSGWKISIRGNRIIWTLIDINGKTKSVFFEYNIREDISEYINRWFFVTITNNLNNAKIYINGKLESNTDIKDIREVIANGEIIFKLDGDIDRTQFIWMKYFSIFNTELSQSNIEERYKIQSYSEYLKDFWGNPLMYNKEYYMFNAGNKNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFIIRRKSNSQSINDDIVRKEDYIYLDFFNLNQEWRVYTYKYFKKEEEKLFLAPISDSDEFYNTIQIKEYDEQPTYSCQLLFKKDEESTDEIGLIGIHRFYESGIVFEEYKDYFCISKWYLKEVKRKPYNLKLGCNWQFIPKDEGWTE

Properties

MW: 150,803.64 Da
No. of AA: 1291
Abs 0.1% (g/L): 1.313
pI: 5.23
NCBI ID: WP_012291519.1
UniprotKB: B1INP5
Strains: CN 5009, BL 143, ATCC 2743, 4301B, 5009, Okra, BL 150, Colworth BL147, CN 7242, Colworth BL86, NCTC 7273, NCIB 4301, CDC 15044, FT 239, CN 5009 (2), BL 143 (2), NCTC 7273 (2), DFPST0017, DFPST003, DFPST0006, DFPST005, DFPST002
Associated Proteins: NTNH/B1 (E014).
Notes:

Associated Publications

Genetic diversity among Botulinum Neurotoxin-producing clostridial strains.
Hill KK, Smith TJ, Helma CH, Ticknor LO, Foley BT, Svensson RT, Brown JL, Johnson EA, Smith LA, Okinaka RT, Jackson PJ, Marks JD. (2007). J Bacteriol.
DOI: 10.1128/JB.01180-06   PMID: 17114256

Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence.
Whelan SM, Elmore MJ, Bodsworth NJ, Brehm JK, Atkinson T, Minton NP. (1992). Appl Environ Microbiol.
DOI:   PMID: 1514783

Structure of dual receptor binding to botulinum neurotoxin B.
Berntsson RP, Peng L, Dong M, Stenmark P. (2013). Nat Commun
DOI: 10.1038/ncomms3058   PMID: 23807078

Augmentation of VAMP-catalytic activity of botulinum neurotoxin serotype B does not result in increased potency in physiological systems.
Elliott M, Maignel J, Liu SM, Favre-Guilmard C, Mir I, Farrow P, Hornby F, Marlin S, Palan S, Beard M, Krupp J. (2017). PLoS ONE
DOI: 10.1371/journal.pone.0185628   PMID: 28982136

Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors.
Tao L, Peng L, Berntsson RP, Liu SM, Park S, Yu F, Boone C, Palan S, Beard M, Chabrier PE, Stenmark P, Krupp J, Dong M. (2017). Nat Commun
DOI: 10.1038/s41467-017-00064-y   PMID: 28674381

A Cell Line for Detection of Botulinum Neurotoxin Type B.
Rust A, Doran C, Hart R, Binz T, Stickings P, Sesardic D, Peden AA, Davletov B. (2017). Front Pharmacol
DOI: 10.3389/fphar.2017.00796   PMID: 29170639

Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models.
Elliott M, Favre-Guilmard C, Liu SM, Maignel J, Masuyer G, Beard M, Boone C, Carré D, Kalinichev M, Lezmi S, Mir I, Nicoleau C, Palan S, Perier C, Raban E, Zhang S, Dong M, Stenmark P, Krupp J. (2019). Sci Adv
DOI: 10.1126/sciadv.aau7196   PMID: 30746458

Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B.
Flores A, Ramirez-Franco J, Desplantes R, Debreux K, Ferracci G, Wernert F, Blanchard MP, Maulet Y, Youssouf F, Sangiardi M, Iborra C, Popoff MR, Seagar M, Fantini J, Lévêque C, El Far O. (2019). Proc. Natl. Acad. Sci. U.S.A.
DOI: 201908051   PMID: 31431523

Structural and Biochemical Characterization of Botulinum Neurotoxin Subtype B2 Binding to Its Receptors.
Davies JR, Masuyer G, Stenmark P. (2020). Toxins (Basel)
DOI: E603   PMID: 32957706

Is your publication missing from the database? Add it here.